" Hi, my name is frans.
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Abstract

Nico A.J. van Nuland, Alard A. van Dijk, Klaas Dijkstra, Frans van Hoesel, Ruud M. Scheek and George T. Robillard
Three-dimensional 15N-1H-1H and 15C-13C-1H nuclear-magnetic resonance studies of HPr, a central component of the phospoenolpyruvate-dependent phosphotransferase system for Escherichia coli. Eur.J.Biochem. 203 (1992) 483-491

We have performed three-dimensional NMR studies on a central component of the phosphoenolpyruvate-dependent phophotransferase system of Escherichia coli, denoted as HPr. The protein was univormly enriched with 15N and 13C to overcome spectral overlap. Complete assignments were obtained for the backbone 1H, 15N and 13C resonances, using three-dimensional heteronuclear 1H NOE 1H-15N multiple-quantum coherence spectroscopy (3D-NOESY-HMQC) and three-dimensional heteronuclear total correlation 1H-15N multiple-quantum coherence spectroscopy (3D-TOCSY-HMQC) experiments on 15N-enriched HPr and an additional three-dimensional triple-resonance 1HN-15N-13Calpha correlation spectroscopy (HNCA) experiment on 13C, 15N-enriched HPr. Many of the sequential backbone 1H assignments,as derived from two-dimensional NMR studies [Klevit, R.E., Drobney, G.P. & Waygood, E.B. (1986) Biochemistry 25, 7760-7769, were corrected. Almost all discepancies are in the helical regions, leaving the published antiparallel beta-sheet topology almost completely intact.